pc 12 cell culture medium  (Procell Inc)

Journal: Frontiers in Cellular Neuroscience

Article Title: CircFRRS1 drives neuroinflammation through the miR-27a-3p/TLR4 pathway after deep hypothermic circulatory arrest

doi: 10.3389/fncel.2026.1750887

Figure Lengend Snippet: Inhibition of circFRRS1 alleviates the inflammation damage of PC-12 cells after H-OGD/R treatment. (A) Sequence diagram of circFRRS1. (B) The inhibition effects of si-circFRRS1 were detected by RT-PCR. (C) CCK8 assay revealed circFRRS1 inhibition improved the survival rate of H-OGD/R treated PC-12 cells. (D–F) The inhibition of circFRRS1 reduced the increasing level of TNF-α, IL-6, and IL-1β induced by H-OGD/R treatment. (G) The inhibition of circFRRS1 alleviated the NLRP3 inflammasome activation of H-OGD/R treated PC-12 cells. *** p < 0.001, vs. si-NC group; ## p < 0.01, ### p < 0.001, vs. H-OGD/R + si-NC group.

Article Snippet: Briefly, PC-12 cells were placed in a 16°C environment with glucose-free Dulbecco’s modified Eagle’s medium (DMEM) without FBS in an incubator containing 95% N2 and 5% CO 2 for 2 h. Then the medium was replaced with PC-12 cell culture medium (CM-0481, Procell) and cells were transferred to the incubator set at 37°C with reoxygenation for 24 h. Finally, the cells were harvested for further experiment.

Techniques: Inhibition, Sequencing, Reverse Transcription Polymerase Chain Reaction, CCK-8 Assay, Activation Assay

Journal: Frontiers in Cellular Neuroscience

Article Title: CircFRRS1 drives neuroinflammation through the miR-27a-3p/TLR4 pathway after deep hypothermic circulatory arrest

doi: 10.3389/fncel.2026.1750887

Figure Lengend Snippet: The inhibition of circFRRS1 suppressed TLR4/NF-κB/NLRP3 pathway in H-OGD/R-induced PC-12 cells. (A) WB analysis revealed that the TLR4/NF-κB/NLRP3 axis was activated in H-OGD/R-induced PC-12 cells and markedly inhibited after si-circFRRS1 transfected. (B–G) Statistical analysis of WB results. * p < 0.05; ** p < 0.01, *** p < 0.001, vs. si-NC group; # p < 0.05, ## p < 0.01, vs. H-OGD/R + si-NC group.

Article Snippet: Briefly, PC-12 cells were placed in a 16°C environment with glucose-free Dulbecco’s modified Eagle’s medium (DMEM) without FBS in an incubator containing 95% N2 and 5% CO 2 for 2 h. Then the medium was replaced with PC-12 cell culture medium (CM-0481, Procell) and cells were transferred to the incubator set at 37°C with reoxygenation for 24 h. Finally, the cells were harvested for further experiment.

Techniques: Inhibition, Transfection

Journal: Cells

Article Title: Melatonin Attenuates Spinal Cord Injury in Mice by Activating the Nrf2/ARE Signaling Pathway to Inhibit the NLRP3 Inflammasome

doi: 10.3390/cells11182809

Figure Lengend Snippet: The oxidative damage scavenging effect of melatonin in neuron cells. ( A ) The survival rates of PC12 cells that received different concentrations of melatonin treatment were detected using a CCK-8 kit (n = 8). ( B ) The ROS produced in PC12 cells that received different treatments were detected by ROS staining (n = 6, scale bar = 100 µm). ( C , D ) The mitochondrial membrane potential in PC12 cells treated with drugs was detected by JC-1 staining (n = 8, scale bar = 100 µm). ( E – G ) The contents of SOD and MDA and the activity of GSH-PX were detected using the corresponding kits in PC12 cells treated with different treatment factors (n = 10). Data are shown as the means ± SD; * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The levels of IL-1β and IL-18 were measured in the PC12 cell culture medium using ELISA kits according to the manufacturer’s instructions (Elabscience, China).

Techniques: CCK-8 Assay, Produced, Staining, Membrane, Activity Assay

Journal: Cells

Article Title: Melatonin Attenuates Spinal Cord Injury in Mice by Activating the Nrf2/ARE Signaling Pathway to Inhibit the NLRP3 Inflammasome

doi: 10.3390/cells11182809

Figure Lengend Snippet: Melatonin suppresses the NLRP3 inflammasome in PC12 cells treated with H2O2 by activating the Nrf2/ARE pathway. ( A – I ) Western blot detection and statistical analysis of NLRP3-inflammasome-related proteins NLRP3, caspase-1, ASC, and IL-1β; and antioxidant-stress-related proteins nuclear Nrf2, HO-1, and NQO-1 in PC12 cells from each group (n = 6). ( J , K ) The levels of IL-1β and IL-18 in the supernatant of PC12 cells were determined by ELISA kits (n = 6). Data are shown as the means ± SD; * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The levels of IL-1β and IL-18 were measured in the PC12 cell culture medium using ELISA kits according to the manufacturer’s instructions (Elabscience, China).

Techniques: Western Blot, Enzyme-linked Immunosorbent Assay

Journal: Cells

Article Title: Melatonin Attenuates Spinal Cord Injury in Mice by Activating the Nrf2/ARE Signaling Pathway to Inhibit the NLRP3 Inflammasome

doi: 10.3390/cells11182809

Figure Lengend Snippet: Immunofluorescence double staining of Nrf2 and NLRP3 in PC12 cells treated with H2O2. Immunofluorescence double staining was performed to test the protein level of Nrf2 ( A , B ) and NLRP3 ( C , D ) in PC12 cells from each group (n = 8, scale bar = 100 µm). Data are shown as the means ± SD; * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The levels of IL-1β and IL-18 were measured in the PC12 cell culture medium using ELISA kits according to the manufacturer’s instructions (Elabscience, China).

Techniques: Immunofluorescence, Double Staining